Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Multifaceted Beneficial Associations with Pseudomonas and Rhizobia on Growth Promotion of Mucuna pruriens L

D.K. Maheshwari1*, Abhinav Aeron1, R.C. Dubey1, Mohit Agarwal1, Shrivardhan Dheeman1 and Shuti Shukla2*

1Department of Botany and Microbiology, Gurukul Kangri University, Hardwar - 249404, India. 2Department of Food Science and Technology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.

Received on 10 July 2014 and accepted on 20 September 2014

 

ABSTRACT

Experiments were conducted to isolate Pseudomonas compatible with both slow and fast growing rhizobia and assessed them for increased vegetative growth in Mucuna pruriens. Influence of cell-free culture filtrate of P. fluorescens PRS4 on growth promotion of M. pruriens in co-inoculation with S. meliloti RMP66rif+ and Bradyrhizobium sp. BMP17kan+ was statistically significant as compared to whole bacterial cultures and washed cells (71.7%, 64.2 % and 68.4 %, respectively, over control set of treatment), suggesting that plant growth promoting attributes in P. aeruginosa PRS4 are both intracellular and extracellular in nature. Synergistic rhizobacteria and rhizobia proved an effective strategy for co-inoculation which contributes multiple benefits such as increased germination, biomass and enhanced nodulation to improve plant health. Productivity of medicinal legumes has been ignored with respect to their use in indigenous and folk system of medicine; hence this study increases the scope of PGPR application from conventional fields.

Keywords : Fluorescent pseudomonads, rhizobia, co-inoculation, microbial interaction