Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Application of RSM to Optimize Medium for Exo-Polysaccharide Production by Bacillus sp

R. Vidhyalakshmi* and C. Valli Nachiyar

Department of Biotechnology, Sathyabama University, Rajiv Gandhi Salai, Chennai - 600 119, India.

Received on 18 February 2012 and accepted on 21 April 2012

 

ABSTRACT

The combined effect of various media components for exo polysaccharide production by Bacillus spp was studied using RSM. The extracted polysaccharide showed good anti tumor activity and an attempt was made to increase the production of EPS using RSM .The temperature (30°C±1°C ) and pH value (7.0± 0.1) were kept constant throughout the experimental time. A conventional method was used to fix the best yielding substrate and Box Behnken Design was employed to design the composition of the media components. The optimal value for the critical components was obtained as follows. Sucrose -30 gm, Yeast extract -2 gm ,Calcium chloride - 4.3gm,Casamino acid -10ml/l with the predicted value of the model 10.02gm/l ±0.001. A quadratic polynomial analysis was applied for predicting the various responses of media components. Under an experiment the yield of exo polymer was 10gm/l ± 0.001. The determination coefficient (R2) was 0.9813 that ensures adequate credibility of the model.

Keywords : Optimization , Box-Behnken design, Exopolysaccharide, Response surface methodology.