Journal of Pure and Applied MicrobiologyVol. 2 No. 1

A Novel Spectrophotometric Method for Determination of Propyl Gallic Acid by Oxidative Coupling with Orcinol

c. Bala Sekharan¹*, C. Rambabu², B. Praveen kumar³ and D. Ravi Shankar4

¹Department of Biotechnology, P.B.Siddhartha College of Arts & Science, Vijayawada - 520 010. ²Department of Biochemistry, Acharya Nagarjuna University, Post Graduate center, Nuzvid-521 201. ³Department of Biochemistry, P.B.Siddhartha College of Arts & Science, Vijayawada - 520 010. 4Department of Biochemistry, Vananchal Dental College, Jarkhand, India.

Received on 08 February 2008 and accepted on 16 March 2008

 

ABSTRACT

A simple, sensitive and reproducible spectrophotometric method is developed for the determination of propyl gallic acid. This method is based on oxidative coupling reaction between propyl gallic acid with orcinol in the presence of Hydrogen peroxide and enzyme horseradish peroxidase to produce colored chromogen (lmax at 550 nm). Results of analysis were validated statistically and by recovery studies. This method is successfully employed for the determination of propyl gallic acid in oils.

Keywords : Propyl gallic acid, orcinol, Visible Spectrophotomety, Beer’s Law.