Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Characterization and Antifungal Activity of Siderophore Produced by Rhizospheric Pseudomonas fluorescence against Fungal Pathogen of Soybean and Groundnut

Syed Sajeed Ali and N.N. Vidhale

Department of Microbiology, Shri Shivaji Science College Amravati, Shivaji Nagar, Morshi Road, Amravati - 444 603, India.

Received on 25 April 2011 and accepted on 03 June 2011



In the present study thirty six pseudomonas species were isolated and identified as Pseudomonas fluorescence from rhizospheric soil of different crops such as sugarcane, groundnut and soybean in different places of Parbhani District. All isolates were shown siderophore production on succinate medium. The maximum siderophore production was found in PFSP03 strain i.e 72%. The strain produced both hydroxamate and chatacholate type of siderophore which was maximum at 10mM iron concentration in the medium. Also antifungal activity was observed against phytopathogens viz., Alternaria alternata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Macrophomina phaseolina.

Keywords : Antifungal Activity, Siderophore, Pseudomonas fluorescence.