Journal of Pure and Applied MicrobiologyVol. 2 No. 1

Induced Resistance in Cucumber Plant Against Cucumber Mosaic Virus by Fungal Isolates

A.Q. Assarany1 and A.M. Galal2

┬╣Department of Biology, Faculty of Science, Taibah University, Kingdom Saudi Arabia. ┬▓Department of Botany, Faculty of Science, Zagazig University, Egypt.

Received on 02 March 2008 and accepted on 18 April 2008



The induction of resistance of Cucumber plants (Cucumis sativa) against cucumber mosaic virus (CMV) by either ethyl alcohol extracts or cultural filtrates of twenty fungal isolates from Al-Madinah AL-Monawarah date palm farms, Kingdom of Saudi Arabia have been investigated( Aspergillus clavatus, A. fumigatus, A. glaucus, A. nidulans, A. niger, A. terreus, A. versicolor, Penicillium expansum, P. funiculosum, P. griseofulvum, P. janczewskii, P. janthinellum, P. nigricans, P. notatum, P. rubrum, P. chrysogenum, Botrytis squamosa, B. byssoidea, Fusarium solani and F. oxysporum). Seeds of cucumber were soaked with either the filterates of cultivated fungi or the ethyl alcohol extracts of these fungi at different periods. An inhibitory effect on the infectivity of CMV was recorded. In vitro and in vivo studies on the antiviral activities of different fungal agents were applied. The purification of the most potent fungal extract (F. oxysporum) was done using DEAE- cellulose column chromatography and the active substance with high antiviral activity was found to be protienacious in structure.

Keywords : Fungal extracts, cucumber mosaic virus, induction of resistance.