Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Inducible Clindamycin Resistance, Glycopeptide Resistance and Mupirocin Resistance in Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Clinical Samples

G.K. Mangala¹, P.N.S. Rao¹, G. Vishwanath¹, K. Suresh¹ and V. Vijayanath²

¹Department of Microbiology, JJM Medical College, Davangere, India. ²Department of Forensic Medicine & Toxicology S.S.Institute of Medical Sciences & Research Centre, Davangere, India.

Received on 20 March 2011 and accepted on 09 May 2011



Staphylococcus aureus, a common pathogen is well known for its multidrug resistance. Existence of MRSA is further worsened by inducible clindamycin resistance and emerging glycopeptide resistance. Our aim of the study was to detect inducible clindamycin resistance, vancomycin resistance and mupirocin resistance among MRSA isolates. One hundred non-repetitive isolates were subjected to routine antibiotic susceptibility testing by Kirby Bauer’s disc diffusion method including cefoxitin disc for MRSA. Inducible clindamycin resistance was detected by D-test, E-test for vancomycin MIC and mupirocin resistance by disc diffusion. Twenty three isolates showed inducible clindamycin resistance, one showed constitutive resistance and three showed MS phenotypes. Inducible clindamycin resistance, constitutive resistance and MS phenotype were found to be higher in MRSA as compared to MSSA. Only one isolate with vancomycin MIC 4µg/ml by E-test was considered as VISA. Forty one isolates were found resistant to mupirocin, which is a cause for concern. Study showed that D-test should be included as routine disc diffusion test to prevent therapeutic failure with clindamycin.

Keywords : Clindamycin resistance, D-test; mupirocin resistance, constitutive MLSB phenotype, inducible MLSB phenotype.