Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Isolation and Identification of Endophytes from Different Cultivars of Rice (Oryza sativa L.) under Wetland and Upland Conditions in South Assam

Folguni Laskar*, Thounaojam Nevita and G.D. Sharma

Microbiology Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar - 788 011, India.

Received on 10 June 2011 and accepted on 25 August 2011

 

ABSTRACT

Diversity of endophytic microbes both fungi and bacteria was evaluated from a wetland rice field in Karimganj district and upland rice field of Cachar district of South Assam. Three most popular varieties of Boro Rice of South Assam were selected for the study. Cladosporium sp, Fusarium sp, Aspergillus sp, Penicillium sp, and Helminthosporium sp., were identified and Aspergillus sp, Herbaspirillum sp, Acetobacter sp, Enterobacter sp and Pantoea sp were the bacterial endophytes identified in all the varieties in both the sites. Acetylene reduction assay showed that all the bacterial isolates are diazotrophic in nature. Comparison of the colonization rate revealed that wetland rice field and Mahsuri variety of rice harbored the highest diversity of endophytes. Maximum diversity of the endophytes was observed in root region followed by stem and leaves.

Keywords : Diversity, Endophytes, Wetland and Upland rice field, Mahsuri variety, Acetylene reduction assay.