Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Isolation, Purification and Characterization of An Extracellular Agarase from Alteromonas sp.

Parvathy R. Chandran and Jaquline Chinna Rani I*

Women’s Christian College, College Road, Chennai – 600 006, India.

Received on 30 June 2011 and accepted on 04 September 2011

 

ABSTRACT

A marine agarolytic bacterial strain of Alteromonas producing an extracellular agarase was isolated from the sea water from Marina and characterized. The optimum growth requirements of the organism for production and activity of the enzyme was determined. The agarase enzyme was purified by the ammonium sulphate precipitation method and dialysis. The molecular weight of the partially purified enzyme was estimated to be 56kDa by NATIVE-PAGE.

Keywords : Agarase, Alteromonas sp, PMSF.