Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Comparative Study of Peripheral Blood Smear and Rapid Diagnostic Kits for Antigen Detection for Diagnosis of Malaria

Rajeshwari R Surpur1*, K.G. Basvarajjappa1, Venkatesh M. Patil2, M.R. Anitha3 and V. Vijayanth4

¹Department of Microbiology, ²Department of Pharmacology, ³Department of Anatomy, 4Department of Forensic Siences, S.S Institute of Medical Sciences and Research Centre, Davangere - 577 005, India.

Received on 12 January 2010 and accepted on 21 February 2010

 

ABSTRACT

Rapid diagnosis of malaria is an important tool for effective treatment & to prevent complication of malaria & also to reduce mortality and morbidity caused by malaria. This study was taken to compare the efficacy of two methods available in our institutional set up i.e. thick & thin smear & Rapid diagnostic test kit to detect pLDH & HRP in blood of patient. A total of 235 samples were collected from patients of pyrexia suspected to be malaria by sign/symptoms for traditional microscopy: Thick & thin smears were prepared & stained with Leishman’s stain, taking thick smears as gold standard. Antigen detection was done using commercially available kits, out of 235 samples thick smear were positive in 46 cases & thin smear in 28 cases & antigen (Malariagen) were positive in 34 cases. Thin smears had a sensitivity & specificity of 60.86 % and100% respectively. Antigen detection had a sensitivity & specificity of 73.91 % and100% respectively. Leishman’s stained thick smear although cost effective, is difficult to interpret unless seen by experienced microscopists & its time consuming & labour some technique were as antigen detection is rapid , simple, nonmicroscopic method , easy to interpret and can be done bedside of patients or during night calls or emergency.

Keywords : Malaria, diagnosis, Antigen detection, sensitivity, specificity, efficiency.