Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Rapid Detection of Salmonella enterica Serovar typhi from Humans

A. Ganeshkumar, U. Shalini, K. Vaishnavi, Remya Ismail, A. Lakshmanaswamy, N.S. Vasanthi and Arunava Das*

Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam - 638 401, India

Received on 28 March 2010 and accepted on 12 May 2010

 

ABSTRACT

Present study was carried out to investigate Salmonella enterica serovar Typhi associated with Typhoid fever in humans. A total of six blood samples were collected from six patients (4 males and 2 females) and processed for the isolation and identification of the causative agents and their virulence determinants. Microbial investigation revealed the causative agents Salmonella enterica Serovar Typhi in all the six clinical cases. In polymerase chain reaction (PCR) assay, all the six isolates were found positive for the Invasion gene (invA; 244bp fragment), Tyvelose epimerase gene (tyv; 615bp fragment), phage-1 flagellin gene for d-antigen (fliC-d; 750bp fragment) and Vi antigen genes (viaB; 439bp fragment). This study confirms the association of virulent strains of Salmonella enterica Typhi in occurrence of the Typhoid fever in humans. Present study suggested that PCR can be a useful, high throughput diagnostic tool for the rapid detection of Salmonella enterica Serovar Typhi.

Keywords : Salmonella enterica Serovar Typhi, PCR, Typhoid, Virulence.