Journal of Pure and Applied MicrobiologyVol. 2 No. 1

Xylanase Production Under Submerged Fermentation by Newly Isolated Bacillus cereus BSA1: Parametric Optimization of Cultural Conditions

A. Mandal, S. Kar, P.K. Das Mohapatra, C. Maity, K.C. Mondal* and B.R. Pati

Department of Microbiology, Vidyasagar University, Medinipur - 721 102, India.

Received on 12 December 200 and accepted on 25 January 2008

 

ABSTRACT

Extracellular xylanase production by the newly isolated Bacillus cereus BSA1 was optimized under submerged fermentation. The growth of bacteria and its enzyme production were increased upto 72 h and after that bacteria turned to its stationary phase but enzyme production reached its maximum level at 84 h. Incubation temperature of 35oC and initial medium pH of 6.0 were found to be optimum for xylanase production. Pure xylan induced higher amount of xylanase production in respect to other tested agricultural wastes. Maximum enzyme production by the organism was obtained with a xylan concentration of 0.5% (w/v). The xylanase production became repressed when readily available monosaccharides like glucose, xylose, arabinose (0.5% w/v) were added to the xylan containing medium. This article sounds for the first time of smart and successful production of xylanase from a mesophilic soil bacterium, Bacillus cereus BSA1.

Keywords : Bacillus cereus, xylanase, optimization, submerged fermentation.