Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Bioprocessing of Paddy Straw for the Production and Purification of Gallic acid using Penicillium chrysogenum

R. Paranthaman¹, S. Murugesh² and K. Singaravadivel¹

¹Indian Institute of Crop Processing Technology, Thanjavur- 613 005, India. ²SASTRA University, Thanjavur - 613 402, India.

Received on 07 April 2010 and accepted on 29 May 2010

 

ABSTRACT

Tannin acyl hydrolase is an industrially important enzyme that is mainly used in the food and pharmaceutical industry. The present work has been taken up with a view of exploring the possibilities of using Agriculture by products for the production of tannase and optimizing condition require to get maximum production using Aspergillus flavus, Trichoderma viride and Penicillium chrysogenum was selected and optimized for Tannase enzyme production in solid state fermentation using cheaper sources of paddy straw. Tannase production has been evaluated using solid-state fermentation (SSF) at different temperatures, pH, and incubation time. Optimum fermentation conditions of pH, temperature and Incubation period for Tannase production were found to be 5.5 and 30°C at 96 hrs. Thus the present study proved that the Penicillium chrysogenum produced tannase shown in crude activity 17.0 (U/g/min), Ammonium sulphate precipitation 27.0 (U/g/min), Dialysis 34.0 (U/g/min), Column Chromatography 43.0(U/g/min) and gallic acid content (0.18 mg/gm )was determined by using HPLC method. The molecular structure of Gallic acid (Rt.6.55 min) was confirmed by using Gas Chromatograpgy with Mass Spectrophotometer (GC-MS) method.

Keywords : Tannase, gallic acid, , Paddy straw, HPLC, GC-MS.