Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Antimicrobial Activities of Selected Plant Extracts from the Guyana’s Flora

R.C. Jagessar* and Nazrana Mohamed

Department of Chemistry, University of Guyana, South America.

Received on 08 April 2010 and accepted on 28 May 2010

 

ABSTRACT

Antimicrobial activities of leaves of selected plants: sweet broom (Scoparia dulcis), sandbox tree (Hura crepitans), orange (Citrus sinensis), papaya (Carica papaya) and soursop (Annona muricata) were investigated using the pour plate method. The dried leaves of the above mentioned plants were exhaustively extracted in dried hexane, dichloromethane and methanol. Solvents were removed in vacuo to yield paste and viscous oil. Each solvent type extract was subjected to antimicrobial activity tests using the pour plate method. Using the number of non existence colonies as an indication of the antibacterial and antifungal properties of the extract, it was found that the methanol extract was the most antibacterial (zero colonies survive) followed by dichloromethane extract and hexane extract. Controls for the bacteria/fungus were tested against the solvents and it was found that the solvent had no antiseptic effect on the microorganisms. Future work involves the isolation and purification of natural products and an investigation of their individual antimicrobial activity.

Keywords : Antimicrobial, selected plants, pour plate, antibacterial, antifungal, antiseptic, microorganisms.