Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Molecular Identification and Classification of Unknown Microorganisms Based on 16s rRNA

D. Satya Ranjan¹*, Y. LakshmiVasavi¹ and J. Shankar²

1Department of Microbiology, Institute of Science, GITAM University, Visakapatnam - 530 045, India. 2HELINI Bio molecules, Guntur, India.

Received on 14 July 2009 and accepted on 12 August 2009

 

ABSTRACT

Traditional microbiological methods creates a indistinct thought about their classification and their taxonomic status. Several DNA-based typing methods provide information for delineating bacteria into different genera and species and have the potential to resolve differences among the strains of a species. Therefore, newly isolated strains must be classified on the basis of the polyphasic approach. also previously classified organisms, as and when required, can be reclassified on this ground in order to obtain information about their accurate position in the microbial world. The complete 16s rRNA gene sequencing and its comparative analysis of phylogenetic trees for determining taxonomic position of microbes. From the comparative study of phylogenetic tree which is a quantitative identification of the number of positions that differ between the aligned macromolecules, our sample microorganisms belongs to Volume 2, The Proteobacteria, Class III gamma (g) proteobacteria.

Keywords : EDTA: Ethylene Diamine Tetra Acetic acid, TE buffer: Tris EDTA buffer.