Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Effect of Certain Anti-diabetic Ayurvedic Drugs Against Microbes Causing Diabetes-Dependent Infections

P. Abhilash1,2, S. Pradeep2, R.B. Smitha2, V.N. Jisha2, S. Balachandran3 and Sailas Benjamin2*

1 Centre for Distance Education, Bharathidasan University, Tiruchirappalli - 620 023, India. 2Biotechnology Division, Department of Botany, University of Calicut, Kerala - 673 635, India. 3Department of Chemistry, N S S College, Cherthala, University of Kerala, Kerala - 688 556, India.

Received on 04 May 2009 and accepted on 07 August 2009

 

ABSTRACT

The ayurvedic drugs Glymin®, Hyponidd®, Madhumerin®, Mersina® and Limit® are administered as anti-diabetic drugs in the Indian system of medicine in which Gymnema sylvestre is a common ingredient. We investigated the antimicrobial properties of the ethanolic extracts of these drugs against common microbes causing diabetes-depended infections using agar-well diffusion method. The zone of inhibition (in the order of high to low) for each drug was: Glymin - S. aureus, S. pyogenes, B. subtilis, Pseudomonas aeruginosa; Limit - B. subtilis, S. pyogenes, S. aureus, Enterococcus faecalis; Mersina - S. aureus, S. pyogenes, B. subtilis, E. faecalis; Madhumerin - S. pyogenes, C. albicans, S. aureus; and Hyponidd - S. pyogenes. The minimum inhibitory concentration of the drugs against the test microorganisms were in the range of 75 – 200 mg ml-1 (1.9 – 5.0 mg well-1). The Gas Chromatograph – Mass Spectrometric analysis of the ethanolic extracts of the drugs revealed the presence of Ar-tumerone and curlone in Madhumerin; palmitic and benzoic acids in Glymin®; tetradecanoic acid, asarone and caryophyllene in Limit®; a-citral in Mersina® and d-elemene in Hyponidd® to a high percentage similarity with the internal standards of the instrument. To sum up, this study suggests that the components present in the polyherbal formulations (Mersina®, Glymin® and Limit®) would play a vital role in inhibiting diabetes-depended infections thereby reducing the gravity of the disease with the advantage of reducing treatment cost and the quantity of drug consumption.

Keywords : Antimicrobial, Ayurvedic drugs, Diabetes, Diabetes-dependent infections