Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Molecular Characterization of Lipase Producer from Local Marine Environment

Syarul Nataqain BaharumĀ¹*, Maya Erna NathanĀ² and Mohd Azinuddin Ahmad Mokhtar1

1 Institute of Systems Biology Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. 2 Faculty of Biotechnology and Life Sciences Jalan Zirkon A7/7, Seksyen 7, 40000 Shah Alam, Selangor, Malaysia.

Received on 30 June 2009 and accepted on 07 August 2009

 

ABSTRACT

Lipolytic bacteria were screened from marine water sample in Pantai Morib, Selangor. Eight out of 100 isolates exhibited positive lipolytic activities via qualitative screening assay using the tributyrin agar plates. The halo formation around bacteria colonies indicates the production of lipase activity. Quantitative assay was performed and isolate S14 exhibited highest lipase production (6.404 U/ml) compared to other isolates. The Polymerase Chain Reaction (PCR) technique was employed for the 16S rDNA. The 16S rDNA fragment with 1500 bp in size was cloned and sequenced. However, there was only 1004 bp of 16S rDNA nucleotides was successfully sequenced. Isolate S14 was identified as Bacillus sp. based on 95% homology with the reference sequences of Bacillus sp. from the GenBank. Based on these results we conclude that lipase producer was successfully isolated from the local marine environment and identified as Bacillus sp.

Keywords : Lipase, Marine bacterium, 16S rDNA, Bacillus sp.