Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Genetic Diversity of 41 Countries Phaseolus vulgaris L. Accessions, by ISSR Technique

Mohamed S. El-Shikh

Department of Botany and Microbiology, College of Science,
King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia

Received on 06 August 2014 and accepted on 31 October 2014



The genetic diversity of 60 Phaseolus genotypes was investigated, which represented 41 countries around the world. Phaseolus vulgaris L. is the world’s second most important bean after soybeans; used for human food and animal feed. ISSR effectiveness in achievement of the genetic diversity among 60 common bean accessions. Into 2 major groups the studied Phaseolus vulgaris accessions were divided. Accessions of Asia and Europe seem to be more genetically similar (99%) to each other as they clustered in the same sub-group. The American and African varieties showed similarities as well and clustered in the same sub-tree group. In contrast, Asian and American accessions No. 22 and 23 showed high level of genetic similarities, although these were isolated from different regions. The phylogenetic tree showed that all the Asian accessions (along with Australian No. 59 and 60) were similar except Indian and Yemen accessions No. 9 and 20. Only Netherlands accession No. 3 was different from the rest of European accessions. Morocco accession No. 52 was genetically different from the rest of the African accessions. Canadian accession No. 44 seems to be different from the other North American accessions including Guatemala, Mexico and USA.

Keywords : Genetic Diversity, Phaseolus vulgaris L., Accessions, ISSR Technique.