Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Multiplex PCR Assay for Detection of VHSV, IPNV and IHNV in Eyed Egg, Fry and Broodstock of Rainbow Trout, Oncorhynchus mykiss, Hatcheries in West Iran

Firooz Fadaeifard1*, Mehdi Raissy1 and Hassan Momtaz2

1Department of Aquatic Animal Health , Faculty of veterinary medicicne, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. 2Department of Microbiology, Faculty of Veterinary Medicicne, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Received on 18 February 2013 and accepted on 21 April 2013

 

ABSTRACT

Infectious hematopoietic necrosis (IHN), viral hemorrhagic septicemia (VHS) and infectious pancreatic necrosis (IPN) are important viral diseases of salmonids especially rainbow trout leading to mass mortality among cultured fish. The aim of this study was molecular detection of IHNV, IPNV and VHSV in eyed egg, fries and broodstock of rainbow trout and comparison of their abundance among hatcheries in the western part of Iran. In this study, 300 eyed eggs, 150 fry and 6 broodstocks were studied in 15 hatcheries. All samples were examined by Reverse Transcriptase Polymerase Chain Reaction assay. The frequency of IHNV, IPNV and VHSV were 37.28, 46.96 and 8.19 percent, respectively as 20.89% of the eggs, 54.44% of fries and 17.14% of the broodstocks found to be infected by one of the viruses. The present study revealed that examined fish, both fry and broodstock, were simultaneously infected by all three kind viruses which is very important epidemiologically.

Keywords : IHN, IPN, VHS, rainbow trout, RT-PCR.