Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Biological Activity of Plant Tuber (RVL1) and Fungal Lectin (SRL1) Against Sclerotium rolfsii

K. Jayalakshmi1, S. Lingaraju2, R. S. Bhat3, J. Raju1, S. T. Naik2, S. Raghu4, Y. S. Bhagath3, Madhu S. Giri2 and S. Priti5

1Department of Plant Pathology, University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka 577225, India. 2Department of Plant Pathology, University of Agricultural Sciences, Dharwad, Karnataka 580 005, India. 3Department of Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka 580 005, India. 4Department of Plant Pathology, Central Rice Research Institute, India. 5Department of Plant Pathology, CHES, Chettehalli, Kodagu, Karnataka, India.

Received on 06 June 2015 and accepted on 04 August 2015

 

ABSTRACT

Efficacy of E.coli expressed R. vivipara lectin (RVL1) and S. rolfsii lectin (SRL1) was studied against S. rolfsii. Protein Expression checked through SDS-PAGE, showed respectively an extra protein band corresponding to molecular weight of 28.2 kDa for RVL1 and 19.5 kDa for SRL1. E. coli expressed RVL1 and SRL1 showed agglutination with trypsinised rabbit erythrocytes indicating the presence of lectin. Compared to bacterial control Bl21 and without protein white, smooth, sparse radiating mycelial growth of S.rolfsii was obseved in different concentrations of lectins and the rate of formation of sclerotial bodies increased with increasing concentration of both the lectins. SRL1- treated PDA plates produced bigger, heavier and more number of sclerotial bodies with increasing concentrations of SRL1. Compared to SRL1 treated plates, RVL1 treated plates produced small, lighter and less number of sclerotial bodies. Further, these sclerotial bodies failed to germinate on the medium. This indicates that lectins play a major role in early formation sclerotilal bodies of lectins but inhibited their germination.

Keywords : Plant Remusatia vivipara lectin, fungal Sclerotium rolfsii lectin,