Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Sequence Analysis and Molecular Characterization of mpb83 and cfp2 Immunodominant Genes in Indian Field Strain of Mycobacterium bovis

Ramane Sangram Pandit*1, Rishendra Verma1, Tista Mondal1 and Vikramaditya Upmanyu2

1Mycobacteria Laboratory, Division of Bacteriology and Mycology, 2Division of Biological Standardization, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly - 243122, India.

Received on 20 June 2015 and accepted on 19 August 2015

 

ABSTRACT

The mpb83 and cfp2 genes encoding MPB83 and CFP2 immunodominant proteins were amplified from Mycobacterium bovis field strain 3/86 by polymerase chain reaction. The recombinant plasmids were constructed via inserting the mpb83 and cfp2 genes into the pET vector and named pET28b-mpb83 and pET28b-cfp2, respectively. The nucleotide sequences of the insert PCR products showed an open reading frame of 600 and 360 base pairs for mpb83 and cfp2, respectively. The sequenced genes and its deduced amino acid sequences were compared with the published sequences of reference strains. The sequences of the mpb83 and cfp2 genes share more than 99% similarity at both nucleotide and deduced amino acid level with published reference M. tuberculosis complex strains indicating their conserved nature.

Keywords : Mycobacterium bovis, mpb83 gene, cfp2 gene, sequencing, homology.