Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Extended Spectrum and AMPC Beta-lactamases in the Enterobacteriaceae Isolated from Pediatric Urinary Tract Infection Cases

Anjana Telkar1, C. Baragundi Mahesh2 and M. Rathod Bharati

1J.J.M. Medical College, Davangere, India. 2S.N.M.C.Bagalkot, India.

Received on 10 July 2015 and accepted on 24 August 2015

 

ABSTRACT

Urinary tract infection is the third most leading cause of infection in pediatric age group, causing high morbidity and mortality. Resistance to broad spectrum beta lactams mediated by extended spectrum beta lactamase and AmpC beta lactamase enzymes is an increasing problem worldwide. Unfortunately the ESBL producers often also have resistance determinants to other antibiotic groups, leaving an extremely limited range of effective agents. Study was conducted on 100 non repetitive mid stream urine samples collected in a sterile container from pediatric patients suspected of urinary tract infection. All enterobacteriaceae isolates were subjected for detection of ESBL and AmpC beta-lactamases. 72 enterobacteriaceae were isolated in the study. Among them ESBL producers were 47(65.27%), pure AmpC producers were 23(31.94%) and 31(43.05%) were co-producers (ESBL+AmpC). In the present study, E.coli was the commonest isolate causing UTI in children with highest production of Amp C. Klebsiella spps showed highest production of ESBL. Simple disc tests can be used to detect ESBL and Amp C beta lactamases especially where resources for molecular detection are not available. Many of these beta-lactamases disseminate rapidly being plasmid mediated. Hence it is necessary that rapid detection of these enzymes should be done and immediate control measures should be implemented to prevent their dissemination.

Keywords : ESBL, Amp C, Resistance, UTI.