Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Isolation and Identification of Volatile Metabolites from the Biocontrol Agent Trichoderma harzianum through GC-MS Analysis

Sonika Pandey, Mukesh Srivastava, Mohammad Shahid, Anuradha Singh, Shubha Trivedi, Vipul Kumar, Manoj Kumar Maurya and Yatindra Kumar Srivastava

Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur - 208 002, India.

Received on 03 August 2015 and accepted on 10 October 2015



Trichoderma belongs to a group of filamentous fungi, is an effective biocontrol agent. There are several antagonistic mechanisms exercised by Trichoderma species such as nutrient competition, antibiotic production and mycoparasitism. Use of biocontrol agents for the management of phytopathogens is an economical, ecological and environmental friendly process. The present study involves the isolation and characterization of volatile secondary metabolites from the culture filtrate of Trichoderma harzianum (Th. Azad). The in vitro assay of extracted volatile metabolite of Trichoderma harzianum (Th. Azad) shows growth inhibition of all the tested phytopathogens (Rhizoctonia bataticola 24%, Fusarium oxsporum ciceri 29.36%). The inhibition varied from species to species. Highest growth inhibition was achieved with Fusarium oxsporum ciceri (29.36%). Identification and profiling of volatile metabolites produced by T. harzinum yielded 15 compounds with 15 sharp peaks. Out of the different compounds obtained, 1H-Purine-6-amine (2-Fluro Phenyl) methyl), is first time found to be present in Trichoderma harzianum (Th. Azad). This compound works as a plant growth regulator.

Keywords : Trichoderma, Secondary metabolites, Antagonistic, Volatiles metabolites.