Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Investigation of Antimicrobial Activity and Phytochemical Analysis of Thorns of Ceiba petandra Plant

N.H. Ramli, N.A. Ibrahim, N. Chik and N.H. Mat Yasin

Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, 26300, Gambang, Pahang, Malaysia.

Received on 10 September 2015 and accepted on 10 November 2015

 

ABSTRACT

Thorns of Ceiba petandra or ?kekabu? plant are widely used in complementary medicine and have been recommended for the treatment of many diseases such as bronchitis, diarrhoea and skin disease. The extraction process of this plant (thorn part) was investigated at various conditions such as different types of solvents and solid to solvent ratio during the extraction process. The research attempts to get higher yield of the extracted oil and at the same time to ensure that all important phytochemicals such as flavanoids, alkaloid and tannin are presence in the extracted oil sample. In addition, the antimicrobial activity against Escherichia coli and Staphylococcus aureus were also observed. It is found that the optimum condition is obtained from the sample which extracted at 1:15 using methanol as solvent. Based on the Gas Chromatography Mass Spectrometry (GC-MS) analysis, it was proved that the extracted oil by using methanol contain high amount of active compound compare to sample extract using ethanol due to the polarity of the solvents. Results showed that the methanol extract gave higher degree of inhibition zone than ethanol extract against Escherichia coli and Staphylococcus aureus.

Keywords : Ceiba petandra, phytochemical screening, optimum condition, antimicrobial.