Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Specific and Quick Detection of Ralstonia solancearum (E.F Smith) Yabuuchi et al, from Infected Rhizomes Causing Bacterial Wilt of Ginger by using PCR based Amplification of fliC Gene Coding for the Flagella Subunit

I.D. Anand1, M.R. Ravikumar2, S. Raghu3, H. Virupaksha Prabhu1 and M. Ranganath Swamy1

1Department of Plant Pathology College of Agriculture, Dharwad, India. 2Department of Plant Pathology College of Agriculture, Hanumanamatti, University of Agricultural Sciences, Dharwad - 580 005, Karnataka, India. 3Crop protection division ICAR-Central Rice Research Institute, Cuttack - 753 006, Odisha, India.

Received on 11 April 2015 and accepted on 26 June 2015

 

ABSTRACT

The wilt caused by bacteria (Ralstonia solanacearum) is the most devastating pathogen among all the rhizome rot pathogens. An effort was made for quick detection of bacterial wilt pathogen using PCR based molecular techniques. Phylogram results of four RS isolates Revealed that there are two major clusters, cluster A comprising all Ralstonia solanacearum species, A glance towards dendrogram reveals that there was not much diversity among the four isolates of RS. The fliC gene was amplified and an expected size of 400bp it was confirmed the Ralstonia solanacearum flagellin protein (fliC) gene. Fragment of fliC gene sequences of RS-1 (Haveri) and RS-8 (Shimoga) isolates showed 99 per cent similarity with NCBI published sequence of RS Strain MR11 plasmid mega plasmid flagellin protein (fliC) gene, partial cds (KF031064.1) and DSM9544 flagellin (fliC) gene, partial cds (AY192724.1) respectively. Dharwad isolate showed 98 per cent similarity with Ralstonia solanacearum strain RCR-226 flagellin protein (fliC) gene, partial cds (KC834785.1). While Uttara Kannada isolate showe 97 per cent similarity with RS flagellin (fliC) gene, complete cds (AF283285.1).

Keywords : Ralstonia solanacearum, Bacterial wilt, quick detection, fliC gene, Polymerase chain reaction.