Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Biotransformation of Pentachlorophenol to Intermediary Metabolites by Bacteria Isolated from Pulp and Paper Mill Effluent Irrigated Cultivable Land

Dharmendra Kumar, Rajeev Kaushik, Lata and Anil K. Saxena

Division of Microbiology, Indian Agricultural Research Institute, New Delhi- 110012, India.

Received on 09 August 2015 and accepted on 01 October 2015

 

ABSTRACT

Bacteria capable of utilizing Pentachlorophenol as sole C source in minimal medium were analysed for their capability to biotransform PCP to intermediate metabolites. Selected isolates were also assessed, alone and in combination, for their ability to biotransform PCP in sterile soil. HPLC studies revealed decrease in the residual PCP and formation of intermediates such as tetrachlorohydroquinone (TeCH) and dichlorophenols (DCP) by the isolates. Removal of PCP from sterile soil by selected isolates, viz. Ensifer adhaerens (LK 4), Pseudomonas putida (LK 39) and Lysinibacillus fusiformis (LK156), was studied in a 30 d experiment under controlled condition. These isolates were used alone and in combination. PCP removal by mixed inoculum was significantly higher (68.81%) than single inoculation after 30 d of incubation. The results of this study indicate that the enriched mixed inoculum has better potential in scavenging PCP from soil as compared to individual isolates. The potential isolates obtained in the present study can be used for developing consortium for bioremediation of sites contaminated with PCP.

Keywords : Pentachlorophenol degradation, tetrachlorohydroquinone, Dichlorophenols, HPLC studies, Ensifer adhaerens, Lysinibacillus fusiformis.