Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

In vitro Antagonism of Rhizobium Strains Isolated from Various Legumes

Cigdem Kucuk* and Cenap Cevheri

Harran University, Faculty of Arts and Science, Department of Biology, Sanliurfa, Turkey.

Received on 08 January 2015 and accepted on 24 March 2015



The effects of 20 Rhizobium strains isolated from Phaseolus vulgaris L., Trifolium repens var. repens, Cicer arietinum L., Lens culinaris Medik., Vigna unguiculata L. and Phaseolus vulgaris L. ‘Red Kidney’ plants on mycelium growth of Fusarium oxysporum, F. moniliforme, F. solani, F. culmorum, F. oxysporum F1 strain, F.oxysporum F2 strain, F.oxysporum F3 strain, F.oxysporum F4 strain and Trichoderma harzianum strains were observed on solid medium. The most effective Rhizobium strain against the Fusarium species was R. phaseoli 4.5 strain. Rhizobium sp. 3.5, R. trifolii 2.3a, R. trifolii 2.16 and R. trifolii 2.25a strains didn’t affect the growth of T. harzianum T1. R. phaseoli 1.17, R. phaseoli 1.114, R. ciceri N7 and R. ciceri N28 strains didn’t affect the growth of T. harzianum T15 and R. ciceri N7 strain of Rhizobium didn’t affect the growth of T. harzianum T3. In this study IAA production of Rhizobium strains was assayed colorimetrically. The results showed that IAA production by Rhizobium strains ranged from 2.19 to 129.7 µg ml-1. Phosphate solubilization and siderophore production by the strains were also tested. Amount of IAA, P solubilization and siderophore in R. phaseoli 4.5 strain was the highest. Potential use of this strain needs further tests in planta.

Keywords : Antagonistic effect, Rhizobium, strains, Fungi, in vitro.