Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Characterization of Antibacterial Compounds from Plants by FTIR and GC-MC Analyses

Deepa Madathil* and N. Rajendran

School of Bio Sciences and Technology, VIT University, Vellore, Tamilnadu - 632014, India.

Received on 10 April 2015 and accepted on 08 May 2015

 

ABSTRACT

Biofouling is a critical concern in several environmental conditionsand hence significant economic resources have been dedicated to the development of effective biofouling monitoring and preventive strategies. The present study focused on isolation, characterization and evaluation of compounds from various medicinal and common plants and seaweedwhich are found to be inhibiting biofilm formation, using FTIR and GC-MS. The solvent extracts were scanned by using the KBr pellet method and the characteristic peaks were detected. For GC-MS analysis, methanol extracts were used. The compound detection employed the NIST Ver. 2.0 library.The FTIR spectrum confirmed the presence of functional groups such as carbonyls, amines, alcohol, alkene and aromatic compounds in different plant and seaweedextracts. The results of the GC-MS analysis provided different peaks determining the presence of several compounds with different therapeutic activities. Thus this study creates a platform to screen many bioactive components to act as a biofouling agent.

Keywords : Environmental biofilms,Inhibition, GC-MS, FTIR, Cluster analysis