Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Genetic Analysis of Gene Responsive to Tuberculosis In Under Developing Areas of Pakistan

Afshan Kaleem1*, Farhana Munir1, Mehwish Iqtedar1, Roheena Abdullah1, Fakhar-un-Nisa2, Irfana Iqbal3, Faiza Saleem1, Mahwish Aftab1 and Shagufta Naz1

1Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan 2Department of Zoology, Lahore College for Women University, Jail Road, Lahore, Pakistan 3Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan.

Received on 11 March 2015 and accepted on 27 March 2015

 

ABSTRACT

Tuberculosis is a global infectious disease caused by the bacteria Mycobacterium tuberculosis (Mtb). One third of the world population is estimated to be victim of this bacterial disease with approximately 1.7 million deaths yearly. In this study the presence of Mtb was analyzed in suspected tuberculosis (TB) patients and the percentage in the gender of rural population was calculated. TB was determined by sputum analysis in acid fast structures such as Mycobacterium tuberculosis. The TB positive patients were further investigated for the detection single nucleotide polymorphisms (SNPs) of chemokine ligands C-C motif (CCL-1). CCL1 has been associated with pulmonary TB. Specific primers for SNP (rs12600717 and rs12603965) were designed for CCL1 and amplified by PCR. The amplified gene was run on agarose gel for determining the size of the gene and run with the known molecular weight size markers. In order to verify mutations in CCL1, the amplified gene was sequenced. Four novel mutations were detected, which gave various protein products. These results may lead to development of new diagnostic markers for TB pathogenesis

Keywords : Pulmonary tuberculosis, sputum analysis, CCL1, SNP, novel mutations.