Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Isolation and Identification of Soil Mycoflora from Two Different Brassica campestris L. Fields

Seemal Vehra1, Nazish Noor1, Roheena Abdullah2, Nafeesa Farooq1 and Ejaz Mahmood Ahmad Qureshi3

1Department of Botany, Government Postgraduate College for Women, Samanabad, Lahore. 2Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan. 3Institute of Public Health, Lahore.

Received on 23 April 2015 and accepted on 06 May 2015

 

ABSTRACT

Soil mycoflora was isolated and identified from two different Brassica campestris fields along Multan Road and Raiwind Road, Lahore, Pakistan, to assess the common occurring species. From both sites, a total of 8 species belonging to 3 genera of fungi were isolated. The soil dilution technique was used for the isolation of fungal species from the soil samples and culturing was carried out on Potato Dextrose Agar and Malt Extract Agar medium supplemented with suitable antibiotics such as chloromycitin in order to avoid bacterial contamination. Identification and characterization of the mycoflora up to species level were made on the basis of macroscopic and microscopic morphology such as colony color and texture, and shape of conidia and conidiophores. The most commonly found species which were isolated and identified included Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus nidulans, Aspergillus terreus, Aspergillus oryzae, Fusarium oxysporum and Candida albicans. Aspergillus was found to be the dominating genus in this study. The results obtained from the comparison of mycoflora from the two sites showed that Aspergillus species and Candida albicans were common in both Brassica campestris fields.

Keywords : Mycoflora, soil, Brassica campestris.