Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Variables affecting the Recombinant Phytase Production from Escherichia coli DH5a

Rafidah Mohd Ariff1, MohdYazidAbd. Manap1, Aini Ideris2, Azhar Kassim3, Abd-El Aziem Farouk4 and Anis Shobirin Meor Hussin1*

1Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. 2Department of Science Clinical Study, Faculty of Veterinary Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia 3Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. 4Department of Biotechnology, Faculty of Science, Taif University, P. Box 888, AlHawiya, Kingdom of Saudi Arabia.

Received on 20 November 2014 and accepted on 07 January 2015

 

ABSTRACT

Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombinant phytase production from Escherichia coli DH5. Through the application of the Fractional Factorial Design, the variables of seed age, inoculum level, time of induction, L-arabinose concentration and post-induction time were highly significant for the recombinant phytase production as their p-values were less than 0.05.Thus, minimal changes of this factor mat effect recombinant phytase production from E. coli DH5.

Keywords : Screening; Variables, Optimization,Recombinant Phytase, Fractional Factorial.