Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Composite Treatment of Ectomycorrhizal Fungus Scleroderma bovista with Two Mycorrhiza Helper Bacteria Augmented Banj Oak (Quercus leucotrichophora A. Camus) Growth

Anurag Yadav1*, R.C. Dubey2 and Kusum Yadav3

1Department of Microbiology, College of Basic Science & Humanities, S.D. Agricultural University, S.K. Nagar, Gujarat -385 506, India. 2Department of Botany and Microbiology, Gurukula Kangri University, Haridwar, Uttarakhand- 249 409, India. 3Department of Biochemistry, University of Lucknow, Lucknow - 2260 07, India.

Received on 20 August 2014 and accepted on 14 September 2014

 

ABSTRACT

Effect of composite inoculation of ectomycorrhizal fungi Scleroderma bovista along with two mycorrhizosphere bacterial inoculants was studied on banj oak (Quercus leucotrichophora A. Camus) plant growth in nursery glasshouse experiment. Seven treatments comprising of uninoculated control, two bacterial controls, one ectomycorrhizal (ECM) fungal control, two combinations of mycorrhizosphere bacteria and ECM fungus along with one composite inoculation of two bacteria with ECM fungus were used in the study. Dual treatment of S. bovita with Pseudomonas fluorescens MB9 yielded maximum shoot length (15.26 cm) and collar diameter (0.320 cm) of the plant. Triple inoculation of S. bovista with the two bacteria yielded maximum root length (36.86 cm), lateral roots (24.8), short roots (351.6), dry weight (6.746 g) and ectomycorrhizal colonization (39.2%). The study suggests the use of composite inoculation of native mycorrhizosphere bacteria like Bacillus subtilis, P. fluorescens and others with Scleroderma bovista for the better growth of Q. leucotrichophora, which is a slow growing plant.

Keywords : Growth enhancement, Scleroderma bovista, mycorrhiza helper bacteria, ectomycorrhiza.