Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Molecular Detection of Yellow Mosaic Virus Infecting Legumes

D. Ashwini

Department of Plant Pathology, JNKVV, Jabalpur, India.

Received on 06 August 2014 and accepted on 10 September 2014

 

ABSTRACT

The DNA of whitefly transmitted (WFT) geminivirus was amplified from a viral template present in infected leaves by Polymerase Chain Reaction (PCR) by using degernative primers (PBL1v2040 and PCRc154)that amplify viral DNA-B fragment of approximately500-650bpfor detection of mungbean yellow mosaic virus.

Keywords : Legumes, Polymerase Chain Reaction, degenerate primers.