Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Isolation and Characterization of Lytic Bacteriophage (jSTIz1) against Salmonella enterica serovars Typhimurium

P. Kerketta1*, R.K. Agarwal2, M. Rawat3, L. Jain3, P. Pavan Kumar1, H. Dhanze1, M. Suman Kumar1 and A. Kumar1

1Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar - 243 122, India. 2Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar - 243122, India. 3Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar - 243122, India.

Received on 13 July 2014 and accepted on 12 September 2014

 

ABSTRACT

One new bacteriophage against Salmonella Typhimurium designated jSTIz1 was isolated from sewage of poultry farm located at Bareilly, Uttar Pradesh. Ultrastructural (Transmission Electron Microscopy; TEM) analysis revealed that phage had round head with diameter of 52.95?3 nm, and long, noncontractile tail with length of 190.28?5 nm indicating that it belonged to the order Caudovirales and family Siphoviridae morphotype B2. The isolated bacteriophage was found to be double-stranded DNA. SDS-PAGE profile of bacteriophage revealed four structural proteins ranging from 59 KDa to 16 KDa. Host range analysis indicated that the bacteriophage was lytic to 9 of the 12 isolates of Salmonella tested and did not show lytic activity against S. Saintpaul, S. Virchow, Staphylococcus aureus and E. coli. The phage was found to be stable between the pH 4 to 11 and temperature between 4oC to 60oC.

Keywords : Bacteriophage, host range, Protein analysis, Salmonella Typhimurium, stability