Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Characterization of Hydrocarbon Degrading Bacteria Isolated from Hydrocarbon Contaminated Soil

Muhammad Ammar Athar1, Ali Akbar2,4*, Yasir Hayat Khan2, Imran Ali3,5, Uqba Mehmood1, Anjum Naseem Sabri1 and Shahida Hasnain1

1Department of Microbiology and Molecular Genetics, Punjab University Quaid-e-Azam campus Lahore, Pakistan. 2Department of Microbiology, Faculty of Life Science, University of Balochistan Quetta 87300, Pakistan. 3Institute of Biochemistry, Faculty of Life Sciences, University of Balochistan Quetta 87300, Pakistan. 4Food Engineering and Bioprocess Technology, School of Environment, Recourses and Development, Asian Institute of Technology, Klong Luang, Pathumthani 12120, Thailand. 5Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science Chulalongkorn University, Bangkok, 10330, Thailand.

Received on 18 September 2014 and accepted on 25 November 2014

 

ABSTRACT

Polycyclic aromatic hydrocarbons (PAHs) are of particular concern due to its toxic and carcinogenic properties. The fate of these PAHs and other associated compounds present in the environment is directly related to abiotic and biotic processes; including oxidation, bioaccumulation and degradation by microbes. This study was conducted for the isolation of bacteria with potential biodegradation abilities. Nineteen different bacterial isolates were identified from soils contaminated with PAHs with biodegradation capabilities of different hydrocarbon compounds (1 %) (Xylene, Benzene, Naphylamine, Diphenylamine) added to nutritional media (L. agar and MM2 Agar). These bacterial isolates were found able to grow in the presence of different metals stress along with hydrocarbon at different temperature (25 to 45) and pH (5 to 9) conditions. Majority of the isolates were found belonging to Enterobacteriaceae family, while some of them were from Pseudomonas and Azotobacteriaceae. The bacterial isolates were capable of degrading a verity of different hydrocarbons with its ability to grow in different metals stress environment. Such bacteria can be successfully used for the bioremediation of industrial wastes.

Keywords : Bioremediation, Heavy metals, Industrial wastes, Environmental health, Biodegradation, Public health.