Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Hepatoprotective Effect of Curcumin against Diethyl Nitrosamine Induced Hepatocellular Carcinoma in Albino Rats

Naif Mohammed Kadasa1, Haytham Abdallah2, Mohamed Afifi1,2 and Salah Gowayed1,3

1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, PO Box 11508, Jeddah, 21463, Saudi Arabia. 2Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt. 3Department of Botany, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt.

Received on 12 October 2014 and accepted on 27 November 2014

 

ABSTRACT

Curcumin is widely used traditionally as an old plant in traditional medicine, this work was aimed to investigate its possible protective effect against chemically induced Hepatocellular carcinoma (HCC) in rats. Fifty male albino rats, weighing 120?20g each, were used. Rats were divided to five groups ( n=10) for each group, control group received a single dose of normal saline, diethylnitrosamine (DENA) bearing non-treated group received a single intra-peritoneal dose of DENA (200mg/Kg body weight), the 3rd, 4th and 5th groups were DENA bearing treated group daily administrated curcunine (CUR) via intra-gastric intubation in doses of 300,200 and 100 mg Kg-1 b.wt. respectively for 20 weeks. Serum, and liver samples were used for determination of alpha feto-protein (AFP), Interleukine-2 (IL-2), Interleukine-6 (IL-6), serum liver enzymes (AST, ALT, ALP and GGT) levels as well the activities and gene expression of glutathione peroxidise (GPx), glutathione reductase (GR), catalase (CAT) and super oxide dismutase (SOD). Curcumin was significantly lower the serum level of AFP, IL-2 and IL-6 , ALT, ALT malondialdehyd (MDA) level as well the level of gene expression of IL-2 and IL-6 in contrast increase the levels of gene expression and activities of Gpx, GRD, CAT and SOD. The protective effect of CUR against DEN-induced hepatocarcinogenesis in albino rats was proven.

Keywords : Hepatocellular carcinoma; Curcumin; antioxidant.