Journal of Pure and Applied MicrobiologyVol. 8 No. 6

In vitro Selection and Characterization of Drought-tolerant of some Fig (Ficus carica L.) Plants using DNA Markers

Ehab M.R. Metwali1,2, Hemaid I.A. Soliman3, S.M. Howladar4, H.S. Al-Zahrani1 and M.P. Fuller5

1Biological Science Department, Faculty of Science,King Abdulaziz University, 21589 Jeddah, Saudi Arabia. 2Botany Department, Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egypt. 3Plant Genetic Recourses Department, Desert Research Center,El-Matariya 11753, Cairo, Egypt. 4Biology Department, Faculty of Science, Albaha University, Albaha, Saudi Arabia. 5School of Biological Sciences, Faculty of Science and Environment, Plymouth University, Plymouth, PL4 8AA, UK.

Received on 18 October 2014 and accepted on 03 December 2014

 

ABSTRACT

A preliminary study was conduct to determine the effect of iso-osomatic water deficit on three fig (Ficus carica L.) cultivars using mannitol as a casual agent for drought stress. The shoots of Fig cultivars (i.e., Black Mission, Brown Turkey and Brunswick) were sub-cultured on MS medium supplemented with 3 mg L-1 BAP and 0.5 mg L-1 2iP at different concentrations of mannitol (0.0, 50, 100, 150, 200, 250, and 300 mM L-1) for 35 days under in vitro culture conditions. Number of newly formed shoots, shoot length, leaves number per shoot, necrosis %, fresh weight, dry weight, chlorophyll content, relative water content were determined at regular intervals. The Obtained results showed that all growth parameters were reduced by increasing the mannitol concentration except for the percentage of necrosis % which was increased with a significant difference among all treatments. Black Mission was found to resist Mannitol up to concentration of 300 mM L-1, while Brown Turkey and Brunswick cultivars was found to resist mannitol up to the concentration of 250 mM L-1only , where both of this cultivars was not survived under 300 mM L-1. Genomic DNA of the Ficus carica L cvs. Black Mission, Brown Turkey and Brunswick were extracted and were used in performing Randomly Amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Three arbitrary oligonucleotide primers showed that the number of amplified fragments different from one cultivars to another and this indicating that the fig cultivars are not always identical in their DNA ability to be amplified, which may due to unsimilarity in their genetic structure and common selection history. The use of RAPD molecular markers will be more effectiveness than ISSR to distinguish between the different fig cultivars.

Keywords : Fig (Ficus carica L.), Drought, Mannitol, Proline, Chlorophyll, Polymorphism, Molecular markers.