Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Impact of Microorganisms Strains on Diversity of Epiphytes of Tomato Seeds

Tatiana Melnychuk1, Tatiana Parkhomenko1, Evgenii Andronov2, Volodymyr Patyka3 and Antonina Kalinichenko4,5

1Institute of Agriculture of Crimea, National Academy of Agrarian Sciences, Deputy of director on the advanced study, 150 Kievskaya St., Simferopol, 95453, Ukraine. 2GNU All-Russian Research Institute of Agricultural Microbiology, National Academy of Sciences, head of department, Podbelsky chausse 3, St-Petersburg, Pushkin 8, 196608, Russia. 3D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Science of Ukraine, head of department, 154 Acad. Zabolotny St., Kyiv, D03680, Ukraine. 4 Opole University, Professor of Process Engineering Chair, Poland, 45-365, Opole, Dmovskiego str, 7-9. 5 Poltava State Agrarian Academy, Poltava, Ukraine, 36003, Skovorody Str., 1/3

Received on 11 August 2014 and accepted on 28 September 2014

 

ABSTRACT

Results obtained in our study suggest that introduction of microorganism strains into rhizosphere of plants and epiphytic microflora of tomato seeds causes changes in composition of microbial environment of seed surface resulting in increase in diversity of microorganisms. Inoculation of tomato seeds by strains – bio-agents of bio-fertilizers, namely Azotobacter vinelandii 10702 – Azotobacterin, Enterobacter nimipressuralis 32-3 – Phosphoenterin, Paenibacillus polymyxa P – Biopolicide and Rhizobium radiobacter 10, which is a basis of Agrophyl, causes changes in composition of microbial diversity of seed surface, which is observed during the entire period of storage. Microorganisms are able to stay preserved on the surface of tomato seeds, with the longest period of time observed for sporous strain Paenibacillus polymyxa P. High rate of reproduction of Enterobacter nimipressuralis 32-3 strain at the beginning of the seed storage allows the occupation of the niche for long period of time. Inoculation of seeds with non-spore-forming Rhizobium radiobacter 10 and Azotobacter vinelandii 10702 strains causes similar effect on epiphytes formation, promotes increase in diversity of strains even after 78 months of storage, according to the highest Shennon indices of 1.739 and 1.749, respectively, and the lowest indices of domination - 0.2777 and 0.3614, compared to 0.5297 and 0.7829 in control. We also observed an increase in vigour of tomato seeds obtained from inoculated plants as a result of the influence of strains.

Keywords : microorganisms, strains, tomato seeds, germination, epiphytes, diversity index.