Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Antibacterial and Preliminary Phytochemicals Screening of Theraxicum officinale

Iqbal Hussain1*, Riaz Ullah2, Naser M. AbdElsalam3, Sher Hayat4, Kamal Uddin1, Jafar Khan5, Naseem Akhtar6 and Zahoor Ullah7

1Department of Chemistry, Islamia College University, Peshawar, Khyber Pakhtunkhwa, Pakistan. 2Department of Chemistry, Government College Ara Khel FR Kohat, KP Pakistan 3Riyadh Community College, King Saud University, Riyadh 11437, Saudi Arabia 4Department of Chemistry, Kohat University of Science & Technology, Khyber Pakhtunkhwa, Pakistan. 5Department of Microbiology, Kohat University of Science and Technology, Kohat, KP, Pakistan. 6Department of Chemistry, Bacha Khan University Charsada KPK, Pakistan. 7Department of Chemical Engineering, Universiti Teknologi PETRONAS, 31750 Tronoh, Perak, Malaysia

Received on 19 August 2014 and accepted on 06 October 2014

 

ABSTRACT

The antibacterial activity of different crude extracts of different parts of Theraxicum officinale including roots, stem and leaves and solvents (n-hexane, chloroform, ethyl acetate and methanol) against various human pathogens viz., Salmonella thphi (S. thphi), Escherichia coli (E. coli), Pseudomonas aeuroginosa (P. aeuroginosa). Staphylococcus aureus, were carried out. Among the extracts, methanolic extract of the roots fraction showed a maximum zone of inhibition (18 mm) against S. aureus, followed by leaves extract of maximum zone of inhibition (16 m), while the ethyl acetate fraction of leaves showed (15 mm) maximum zone of inhibition against S. auresus. While low activity of the stem extracts were recorded except for the methanolic extract with a maximum of zone of inhibition (13 mm) against S. typhi. In addition, the phytochemical analyses showed the presence of Phenols, Tannin, Alkaloids, Saponins and Flavonoids.

Keywords : Antibacterial activity, Theraxicum officinale, extracts.