Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Bacterial Production of Xylanase using Nichotina tabaccum Leaf Dust as Substrate

Komal P. Acharya* and Prateek Shilpkar

Department of Microbiology, M.D. Gramseva Mahavidyalaya, Gujarat Vidyapeeth, Sadara, India.

Received on 06 August 2014 and accepted on 29 October 2014

 

ABSTRACT

The xylanase producing bacteria were isolated from soil. Among which three isolates KA01, KA02 and KA03 were used for further studies. From optimization study it was observed that, xylanase production is maximum at pH 5 and 300C when 2% (v/v) inoculum of bacterial isolate KA01 was inoculated for 36 hours at 4% concentration of Nichotina tabaccum leaf dust provided with potassium nitrate (1% w/v) as nitrogen source and xylose as carbon source in nutrient medium under submerged condition at 150 rpm. Isolate KA 02 gives maximum xylanase production at optimized pH of 7, fermentation temperature 25oC, substrate concentration 5%, inoculum size 5%, incubation time 36 hrs, N- source urea and peptone (0.75%) and xylose as sugar source. Bacterial isolate KA03 rpoduced maximum xylanase at pH 7.5, incubation temperature 50oC, concentration of substrate 3%, inoculum size 2%, incubation time 48hrs, N- source potassium nitrate and fructose as sugar. Inoculum age of 12 hrs was found optimum for all the three isolates. Further the xylanase production was found highest (1.77 U/mL) under KA 01 fermented under its optimized conditions compared to other two isolates in their optimized conditions.

Keywords : Fermentative production, optimization, enzyme production, plant leaf dust.