Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Characterization of Newly Isolated Xanthomonads using 16s rDNA; ITS; rep PCR (ERIC, BOX)

Khalid Abdullah Ali AbdelRahim1,2*, El-Sayed Mohamed Soltan2,3 and Klaus Rudolph4

1Botany and Microbiology Department, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia. 2Botany Department, Faculty of Science, Sohag University, Sohag 82524, Egypt. 3Botany department, college of science, Al-Baha University, Saudi Arabia. 4Institute of Plant Pathology and Plant Protection, University of Göttingen, Grisebachstr. 6, D-37077 Göttingen, Germany.

Received on 02 August 2014 and accepted on 06 September 2014

 

ABSTRACT

This study aimed to characterize newly isolated xanthomonads, using different genetics fingerprinting techniques. Rep-PCR fingerprinting (ERIC and BOX), 16S-23S Intergenic Transcribed Spacer-PCR (ITS), 16S rDNA amplification, were used for Xanthomonads strains characterization. By combining the ERIC and BOX PCR data using the UPGMA analysis, all strains from Lobelia and Isotoma were represented in one related group with a similarity coefficient of more than 93%. The strains from Lobelia and Isotoma could be identified as Xanthomonas lobeliae. The HV strains from cotton should be named Xanthomonas axonopodispv. malvacearum race 20. The strains from Catharanthus should be named Xanthomonas axonopodis pv. catharanthi.

Keywords : Xanthomonas species, 16S rDNA, rep PCR.