Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Efficacy of Several Potential Biocontrol Agents against Sclerotinia sclerotiorum

Abdurahman H. Hirad1, Mohamed Elsheshtawi2, Ali H. Bahkali1, Abdallah M. Elgorban1,3 and Shaban R.M. Sayed4

1Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. 2Department of Plant Pathology, College of Agriculture, Mansoura University, Mansoura, Egypt. 3Plant Pathology Institute, Agricultural Research Center, Giza, Egypt. 4Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

Received on 25 September 2014 and accepted on 01 November 2014



In vitro seventeen antagonistic microorganisms were evaluated against phytopathogen Sclerotinia Sclerotiorum by dual culture techniques. After 3 days from incubation, the maximum growth inhibition of the tested pathogen was noticed by Coniothyrium minitans (75.83%) followed by Trichoderma hamatum (63.33%). Also, after 15 days, the highest reduction of mycelial growth of S. sclerotiorum came from C. minitans (91.11%) followed by T. hamatum (85.83%). In case of sclerotia germination, C. minitans and T. hamatum significantly inhibited sclerotia germination with 95.83 and 91.67% reduction, respectively.

Keywords : Sclerotinia sclerotiorum, White rot, Glicoladium catenulatum, Biocontrol.