Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Gas Chromatography-mass Spectrometry (GC-MS) Analysis of Bioactive Compounds Extracted from Streptomyces antibioticus by Ethyl Acetate

Essam N. Sholkamy1,2

1Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia. 2Department of Botany and Microbiology, Faculty of Science, Beniseuf University, Beniseuf, Egypt.

Received on 16 August 2014 and accepted on 27 October 2014

 

ABSTRACT

Sequence of the isolate was submitted in gene bank and provided with accession number KF996507 and named as Streptomyces antibioticus strain ess_amA8. The GC-MS analysis revealed that presence of 2-methyl propanamide, n-hydroxymethylacetamide, hexanamide, 1,2 enzenedicarboxylic acid, hexadecyl-oxirane, 4-hydroxytetradec-2-ynal, 8-pentadecanone, phenylpropanedioic acid, phenylethyl alcohol and 3-methyl-but anamide. Ethyl acetate Extract of Streptomyces antibioticus was most effective against Streptococcus pyogenes ATCC 19615, Staphylococcus aureus ATCC 25923, Klebsiella pneumonia ATCC 70060, Salmonella typhi ATCC 6539 and Pseudomonas aeruginosa ATTC 27853 respectively.The findings obtained from this study may contribute to development of potentially effective and environmentally safer alternative antibacterials.

Keywords : Streptomyces antibioticus,GC-MS, Ethyl acetate, hexadecyl-oxirane.