Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Bacterial Contamination in Cucumber (Cucumis sativus): Evidence from Saudi Arabia

Omar H.M. Shair*, Rasheed M. Al-Ssum and Ashraf A. Hatamleh

Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455 Riyadh 11451, Saudi Arabia.

Received on 10 September 2014 and accepted on 29 October 2014

 

ABSTRACT

The use of waste water in agriculture and aquaculture was sensitive subject. Microbial contaminants in cucumber from the use of sewage water, the air during harvest and transportation stayed in cucumber. Samples were collected from Riyadh outside markets and from supermarkets, washed with tap water, 99% ethanol and sterilized distilled water. Cucumber endocarp sliced in circles and exocarp peels were placed on Mueller Hinton agar, incubated at 37°C overnight. The isolates from the peeled exocarp were Forty one sub cultured and colonies were gram stained. Bacterial colonies were studied morphologically, biochemically and antibiotic susceptibility. The colonies from outside cucumber markets and supermarkets in Riyadh were compared. Sections of the isolated colonies were investigated using scanning electron microscopy. Our study was aimed to identify some pathogenic bacteria in cucumber and provide advice to the consumers in limiting the transmission of infection and consequently help to reduce the risk of infection from eating contaminated cucumber.

Keywords : Contamination, Bacteria, Cucumber peels, Riyadh market, Saudi Arabia, gram staining, SEM, Antibiotic susceptibility.