Journal of Pure and Applied MicrobiologyVol. 8 No. 2

The Biological Influence of Salmonella enterica Lipopolysaccharide on Excretion of Inflammatory Mediators by Fibroblasts in an in vitro Wound Healing Model

Hossein Rastegar1, Hamid Reza Ahmadi Ashtiani2, Shabnam Heydarzadeh Khoyi3, Mehdi Hedayati4, Orkide Ghorban Dadras2, Hediyeh Rassam1, Farinaz Rashed Marandi5 and Siavash Mirzaei6*

1Food and Drug Control Laboratory and Research Center, Ministry of Health and Medical Education, Tehran, Iran. 2Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. 3Student of MSc in Microbiology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. 4Cellular & Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 5Reference Health Laboratory, Ministry of Health, Tehran, Iran. 6R&D Department, SOHA Pharmaceutical Co. Tehran, Iran.

Received on 25 June 2013 and accepted on 20 August 2013

 

ABSTRACT

Inflammatory mediators produced by fibroblasts as the main reagents of wound healing have fundamental roles in this process. Due to stimulatory effects of lipopolysaccharide (LPS) on different biological mechanisms of mammalian cells and its influence on pro-inflammatory cytokines, this bacterial endotoxin has been surge of interest in immunological and inflammatory studies. Skin is the first barrier in immune response which is more susceptible to inflammation. In this investigation, the effects of Salmonella enterica LPS on skin fibroblast cells viability was evaluated. Nitric oxide (NO), cyclooxygenase-2 (COX-2) and hydrogen peroxide (H2O2) levels were assessed after LPS treatment of fibroblasts. Human foreskin fibroblasts were treated by different concentrations of Salmonella enterica LPS (100?g~0.01?g). Effects of LPS on cell viability and NO, COX-2 and H2O2 levels were examined respectively by XTT assay and related kits as per the manufacturer?s protocols. Results of present survey illustrate that there is a dose and time dependent significant difference between control and treated cells in cell proliferation. Results obtained from assays indicate that LPS stimulates NO, COX-2 levels and reduces H2O2 levels in fibroblast cells (p-value<0.001). According to LPS effects on cell proliferation in dose and time dependent manner and increasing nitric oxide as a vital factor in the healing process, it will be considered theraputical potential of this bacterial endotoxin.

Keywords : Salmonella enterica LPS, Inflammation, Nitric oxide, Cyclooxygenase-2, Hydrogen peroxide, Skin fibroblasts, Cell viability.