Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Purification and Characterization of an Extracellular Pectinolytic Enzymes Synthesized by Xanthomonas sp.

Sujata Chavan and M.M.V. Baig*

Department of Botany and Department of Biotechnology, Yeshwant Mahavidyalaya, Nanded - 431 602, India.

Received on 29 November 2013 and accepted on 21 January 2014



Thirty physiologically variant pathogenic strains of Xanthomonas sp from various orchards in and around Nanded district, Maharashtra were isolated. Of the thirty strains, one strain isolated from Citrus lemona from Nanded district, Maharashtra was found to be the most effective in the pectinolytic enzymes production. This strain was subjected for production of pectinolytic enzymes in surface culture and various factors affecting the enzyme production was studied. The optimum time for production of pectinolytic enzymes by this strain was 48 hrs. The optimum temperature and pH for production of pectinolytic enzymes by this strain was 30?c and 7 respectively. The effect of various carbon and nitrogen sources was studied and it was found that pectin was most suitable carbon source and yeast extract was most suitable nitrogen source for optimum production of pectinolytic enzymes.

Keywords : Xanthomonas sps, pectinolytic enzymes.