Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Frequency Distribution of Hepatitis C Virus (HCV) Genotypes and its Association with Viral Loads in Chronic HCV Infected Patients of Isfahan, Iran

Mehrnaz Shanehsazzadeh*1, Javad Sharifi Rad**2,3, Abasalli Pourazar1 and Mandana Behbahani4

1Department of Medicinal Biology, Zist Partak Institute, Academic Center for Education and Research, Isfahane, Iran. 2Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran. 3Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran. 4Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 81746-73441, Islamic Republic of Iran.

Received on 21 March 2014 and accepted on 29 April 2014

 

ABSTRACT

The level of disease proceeding are related to different factors, for example, genotype/subtype and viral load. Six important genotypes of HCV and multiplex subtypes have been determined worldwide, HCV Genotyping is considerable clinical option before planning therapy. The proof is that various genotypes of HCV will need diverse term and of course the anti-viral treatment. The purpose of the current study was to assign the dispensation sample of different genotypes of HCV showed in the research reign and their relationship with the viral load in patients suffering suffer from HCV. Patients having positive serology for HCV were chosen for current research (N=105). After that, viral load and genotyping anticipation were carried out utilizing Real-time PCR 59.05 percent (62/105) of samples were contaminated with genotype 3a and consequently genotype 1a and genotype 1b were detected in 13.33percent (14/105) and 8.57percent (9/105) of samples respectively. However, genotype 3 didn?t deal with a prominently higher viral load in comparison with the other genotypes (P= 0.113). Nevertheless, the prominently higher viral load was detectable among male HCV patients (P=0.012) in contrast to women infected with HCV The current research shows that genotype of HCV 3 computed mostly for almost 70.47 percent of the HCV septicity in Isfahan city. Moreover, genotype 3 was not relevance with more extremity of liver disease in contrast to other genotypes as estimated by viral load.

Keywords : HCV, Genotyping, Viral load, Real time PCR.