Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Molecular Characterization and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Bloodstream Isolated from Alkharj, KSA

Mounir M. Salem-Bekhit1,2, Ibrahim M. Abdel Aziz3,4, Sherif H. Abd-Alrahman5, Ibrahim Alsarra6 and Fares Alanazi1,6

1Kayyali Chair for Pharmaceutical Industries, King Saud University, P. O. Box 2457, Riyadh, Saudi Arabia. 2Microbiology and Immunology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt. 3Medicine Department, Salman Bin Abdul Aziz College of Medicine, Al Kharj, Saudi Arabia. 4Department of Tropical Medicine, Al Azhar Faculty of Medicine, Cairo, Egypt. 5Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University, PO Box, 2455, Riyadh, 11451, Saudi Arabia. 6Department of Pharmaceutics, College of Pharmacy, King Saud University, P. O. Box 2457, Riyadh, Saudi Arabia.

Received on 23 March 2014 and accepted on 02 May 2014

 

ABSTRACT

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired infections in most hospitals worldwide. The aim of this study is to investigate the antimicrobial susceptibility, Panton Valentine Leucocidine (PVL) mecA and toxin genes, agr types and staphylococcal cassette chromosome mec (SCCmec) types of MRSA bloodstream isolates collected from Salman University Hospital, Alkharj, KSA. Antimicrobial susceptibilities were investigated by agar diffusion method; PVL, mecA and toxin genes by polymerase chain reaction (PCR) and SCCmec and agr typing were performed by multiplex PCR. Totally MRSA isolates were susceptible to linezolid and glycopeptide vancomycine. mecA gene was detected in totally of the isolates, on the other hand, PVL positive isolate was not detected. sea was the most frequently (76%) detected enterotoxin gene. SCCmec typing revealed type III in 85% and agr typing revealed type I in 87% of the isolates.

Keywords : Antimicrobial susceptibility, MRSA, Molecular epidemiology, PCR, Toxin gene, mecA, SCCmec.