Journal of Pure and Applied MicrobiologyVol. 8 No. 2

In vitro Evaluation of the Antimycobacterial Activity and Fractionation of Berberis hispanica Root Bark

Amina Cherif Haouat1, Abdellatif Haggoud1, Suzanna David2, Saad Ibnsouda1 and Mohammed Iraqui1

1Universit? Sidi Mohammed Ben Abdellah, Facult? des Sciences et Techniques de F?s. B.P: 2202, F?s, Maroc. 2Instituto Nacional de Sa?de Dr. Ricardo Jorge (INSA,IP), 1649-016, Lisbon, Portugal.

Received on 27 June 2013 and accepted on 18 August 2013



The antimycobacterial activity of the ethanolic extract from Berberis hispanica root bark was evaluated against Mycobact?rium smegmatis and M. aurum. The extract was fractionated and the bands responsible for the activity were identified by bioautography. The results revealed the existence of three of four bands responsible for the antimycobacterial activity of the plant. A phytochemical study evidenced the nature of the main classes of secondary metabolites in the crude extracts and in the active bands of the bioautography. The molecules shown to be responsible for the antimycobacterial activity of the plant included alkaloides, polyphenols and flavonoids.

Keywords : Tuberculosis, Berberis hispanica, antimycobacterial activity.