Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Antimicrobial Resistance and Sensitivity among Isolates of Esherichia coli from Patients in Kahramanmaras, Turkey

Sevil Toroglu1, Hatice Avan1 and Dilek Keskin2*

1Department of Biology, Faculty of Arts and Science, Kahramanmaras S?tc? Adnan University, 46100- Kahramanmaras, Turkey, 2 Cine Vocational High School, Adnan Menderes University, 09500- Cine-Aydin, Turkey.

Received on 10 June 2013 and accepted on 14 August 2013

 

ABSTRACT

To determine the level of resistance to the widely used antibiotics in clinical isolates of Esherichia coli. 74 isolates were collected from different hospitals in Kahramanmaras and recorded at specimens. Antibiotic resistance was determined by agar disc diffusion method using Mueller-Hinton agar according to Clinical and Laboratory Standards Institute recommendations and the production of b-lactamase was detected with the iodometric slide test. This study was carried out in the laboratory of Kahramanmaras S?tc? Adnan University, Biology Department. The results indicated that resistance rate of antibiotics was in the range of 91% Penicilin(PEN), 65% Tetracycline (TET) and Amoxicillin (AMX), 62% Cefazolin(CEFX), 59% Streptomycine( STR), 34% Oflaxain (OFL), 32% Chlorampenicol (CHL) and Ceftriaxone (CEFT), 27% Gentamycin (GEN), 24% Cefoxitin(CEFX), 18% Nitrofurantoin(NIT), %12 Meropenem(MER). Among the 74 isolates of E.coli were showed 38(51%) beta lactamase activity and 36(49%) isolates of E.coli were showed no beta lactamase activity. Out of 74 isolates, 50(68%) isolates showed Multiple Antibiotic Resistance three to twelve antibiotics.

Keywords : Esherichia coli, Antibiotic, Resistance, Beta lactamase activity.