Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Effect of Essential Oils on Growth and Aflatoxin Production by A. parasiticus Isolated from Walnuts

Monira R. Al-Othman1, Abeer R.M. Abd El-Aziz1*, Mohamed A. Mahmoud1,2 and Munirah F. Al-Gahtani1

1Botany and Microbiology Department, College of Science, King Saud University, Riyadh 1145, Kingdom of Saudi Arabia. 2Plant Pathology Research Institute, ARC, Giza, Egypt.

Received on 25 January 2014 and accepted on 29 March 2014

 

ABSTRACT

Effects of five essential oils (Cinnamon, Garlic, Mint, Rosemary and thyme) that seemed to have powerful antifungal and antiaflatoxin characteristics on Aspergillus parasiticus were studied in this research. A. parasiticus is one of the most important species that produces aflatoxin in walnuts. We used three concentrations from each tested essential oils (1,2 ,4). The all tested essential oils appeared more effectiveness on the growth and aflatoxin B and G at three tested concentrations compare to control. Cinnamon oil at 4 % was the highest effective essential oils on inhibition aflatoxin B and G were ranged from 62.7 to 71.0 and 46.4 to 74.4 % and 58.2 to 69.1%, respectively followe by and thyme oil . Mint and Rosemary oil gave moderate effect while garlic oil was the least. As well as aflatoxin was extracted from samples of walnuts then determined by high performance liquid chromatography (HPLC). Their concentrations ranged from 27.8 to 105.1 ?g/kg. The highest levels were found in sample no.4.

Keywords : Essential oils, Aflatoxin, growth, A. parasiticus , walnuts , HPLC.